STUDIES ON THE SUDANESE INDIGENOUS AFRICAN TOAD BUFO SPP. (Amphibia): PARTIAL CHARATERIZATION OF ANTIBACTERIAL PEPTIDES AND PROTEINS OF THE PARATOID GLAND
الملخص
The parotoid gland secretion of Bufo spp., obtained by manual compression, was found to contain 23.1-41.2% (w/w) of total protein. Gel filtration chromatography showed the existence of four fractions of peptides and proteins responsible for antibacterial activity. Thin layer chromatography showed seven Ninhydrin-positive spots in addition to the origin, in the parotoid gland secretion of Bufo spp. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis revealed the existence, in the crude secretion, of 2 bands of proteins (P1 and P2) and 2 bands of peptides (P3 and P4) with various molecular weights.
المراجع
REFERENCES
1. Barberio, C; Delfino, G and Mastromei, G. (1987). A low molecular weight protein with antimicrobial activity in the cutaneous ‘venom’ of the yellow-bellied toad (Bombina variegata pachypus), Toxicon 25 (8): 899-909.
2. Barra, D and Simmaco, M. (1995). Amphibian skin: a promising resource for antimicrobial peptides, Trends in Biotechnology 13 (6): 205-209.
3. Batista, CVF; Scaloni, A; Rigden, DJ; Silva, LR; Romero, AR; Dukor, R; Sebben, A; Talamo, F. and Bloch C. (2001). A novel heterodimeric antimicrobial peptide from the tree-frog Phyllomedusa distincta, FEBS Letters 494 (1-2): 85-89.
4. Bauer, HH; Christian, G.D. and O’Reilly, J.E. (1978). Instrumental Analysis, Allyn and Bacon, Inc. pp: 832
5. Clarke, B.T. (1997). The natural history of amphibian skin secretions, their normal functioning and potential medical applications. Biological Reviews 72: 365-379.
6. Csordás A. and Michl, H. (1969), Primary structure of two oligopeptides of the toxin of Bombina variegata L, Toxicon 7 (2): 103-108.
7. Epand, R.M. and Vogel, H.J. (1999). Diversity of antimicrobial peptides and their mechanisms of action, Biochimica et Biophysica Acta (BBA) /Biomembranes 1462 (1-2): 11-28.
8. Hancock, R.E.W. and Lehrer, R. (1998), Cationic peptides: a new source of antibiotics, Trends in Biotechnology 16 (2): 82-88.
9. Hancock, REW and Chapple, D.S. (1999). Peptide Antibiotic, Antimicrob. Agents Chemother 43 (6): 1317–1323.
Gezira Journal Of Health Sciences 2007 vol.3(1) EDITORIAL
Gezira Journal Of Health Sciences 2007 vol.3(1)
10. Hugo, W.B. and Russell, A.D. (1998). Pharmaceutical Microbiology, sixth edition, Blackwell Science Ltd. pp: 508.
11. Hunter, P. (1977). General Microbiology, the student’s text book, C.V. Mosby company, pp: 366
12. Johnson, E.L. and Stevenson, R. (1978), Basic Liquid Chromatography, Varian Associates, Inc., USA, pp: 354.
13. Kreil, G. (1996). Skin secretions of Xenopus laevis. Pp. 263-277 in The Biology of Xenopus. Symposia of the Zoological Society of London (68). R. C. Tinsley and H. R. Kobel (eds.).
14. Laemmli, U. (1970), Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
15. Lazarus, L.H. and Attila, M. (1993). The toad, ugly and venomous, wears yet a precious jewel in his skin, Prog. Neurobiol. 41 (4): 473–507.
16. Mckay, P. (1999). An Introduction to Chromatography–Seminar, Access
Excellence, The National Health Meusem
17. Nicolas, P. and Mor, A. (1995). Peptides as weapons against
microorganisms in the chemical defense system of vertebrates, Annu Rev Microbiol. 49: 277-304.
18. Perry, D. (2000). Proteins of parotoid gland secretions from toads of the
genus Bufo, Contemporary Herpetology, 2000, 3, ISSN 1094–2246.
19. Rinaldi, A.C. (2002). Antimicrobial peptides from amphibian skin: an expanding scenario: Commentary, Current Opinion in Chemical Biology 6 (6): 799-804.
20. Rödel, M.O. (2000). Herpetofauna of West Africa Vol.1 Amphibians of
the West African Savanna, Edition Chimaira, pp: 332.
21. Rollins-Smith, L.A.; Doersam, J.K.; Longcore, J.E.; Taylor, S.K;
Shamblin, J.C.; Carey, C. and Zasloff, M. (2002). Antimicrobial peptide
defenses against pathogens associated with global amphibian declines,
Developmental and Comparative Immunology 26 (1): 63-72.
22. Rybicki, E. and Purves, M. (1996). SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE), Dept. of Microbiology, University of Cape town.
23. Stahl, E. (1969). Thin-Layer Chromatography. A laboratory handbook, George Allen and Unwin Ltd, pp: 1041.
1. Barberio, C; Delfino, G and Mastromei, G. (1987). A low molecular weight protein with antimicrobial activity in the cutaneous ‘venom’ of the yellow-bellied toad (Bombina variegata pachypus), Toxicon 25 (8): 899-909.
2. Barra, D and Simmaco, M. (1995). Amphibian skin: a promising resource for antimicrobial peptides, Trends in Biotechnology 13 (6): 205-209.
3. Batista, CVF; Scaloni, A; Rigden, DJ; Silva, LR; Romero, AR; Dukor, R; Sebben, A; Talamo, F. and Bloch C. (2001). A novel heterodimeric antimicrobial peptide from the tree-frog Phyllomedusa distincta, FEBS Letters 494 (1-2): 85-89.
4. Bauer, HH; Christian, G.D. and O’Reilly, J.E. (1978). Instrumental Analysis, Allyn and Bacon, Inc. pp: 832
5. Clarke, B.T. (1997). The natural history of amphibian skin secretions, their normal functioning and potential medical applications. Biological Reviews 72: 365-379.
6. Csordás A. and Michl, H. (1969), Primary structure of two oligopeptides of the toxin of Bombina variegata L, Toxicon 7 (2): 103-108.
7. Epand, R.M. and Vogel, H.J. (1999). Diversity of antimicrobial peptides and their mechanisms of action, Biochimica et Biophysica Acta (BBA) /Biomembranes 1462 (1-2): 11-28.
8. Hancock, R.E.W. and Lehrer, R. (1998), Cationic peptides: a new source of antibiotics, Trends in Biotechnology 16 (2): 82-88.
9. Hancock, REW and Chapple, D.S. (1999). Peptide Antibiotic, Antimicrob. Agents Chemother 43 (6): 1317–1323.
Gezira Journal Of Health Sciences 2007 vol.3(1) EDITORIAL
Gezira Journal Of Health Sciences 2007 vol.3(1)
10. Hugo, W.B. and Russell, A.D. (1998). Pharmaceutical Microbiology, sixth edition, Blackwell Science Ltd. pp: 508.
11. Hunter, P. (1977). General Microbiology, the student’s text book, C.V. Mosby company, pp: 366
12. Johnson, E.L. and Stevenson, R. (1978), Basic Liquid Chromatography, Varian Associates, Inc., USA, pp: 354.
13. Kreil, G. (1996). Skin secretions of Xenopus laevis. Pp. 263-277 in The Biology of Xenopus. Symposia of the Zoological Society of London (68). R. C. Tinsley and H. R. Kobel (eds.).
14. Laemmli, U. (1970), Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
15. Lazarus, L.H. and Attila, M. (1993). The toad, ugly and venomous, wears yet a precious jewel in his skin, Prog. Neurobiol. 41 (4): 473–507.
16. Mckay, P. (1999). An Introduction to Chromatography–Seminar, Access
Excellence, The National Health Meusem
17. Nicolas, P. and Mor, A. (1995). Peptides as weapons against
microorganisms in the chemical defense system of vertebrates, Annu Rev Microbiol. 49: 277-304.
18. Perry, D. (2000). Proteins of parotoid gland secretions from toads of the
genus Bufo, Contemporary Herpetology, 2000, 3, ISSN 1094–2246.
19. Rinaldi, A.C. (2002). Antimicrobial peptides from amphibian skin: an expanding scenario: Commentary, Current Opinion in Chemical Biology 6 (6): 799-804.
20. Rödel, M.O. (2000). Herpetofauna of West Africa Vol.1 Amphibians of
the West African Savanna, Edition Chimaira, pp: 332.
21. Rollins-Smith, L.A.; Doersam, J.K.; Longcore, J.E.; Taylor, S.K;
Shamblin, J.C.; Carey, C. and Zasloff, M. (2002). Antimicrobial peptide
defenses against pathogens associated with global amphibian declines,
Developmental and Comparative Immunology 26 (1): 63-72.
22. Rybicki, E. and Purves, M. (1996). SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE), Dept. of Microbiology, University of Cape town.
23. Stahl, E. (1969). Thin-Layer Chromatography. A laboratory handbook, George Allen and Unwin Ltd, pp: 1041.
منشور
2007-01-01
كيفية الاقتباس
ABUGABR, Husam Eldin Elhag et al.
STUDIES ON THE SUDANESE INDIGENOUS AFRICAN TOAD BUFO SPP. (Amphibia): PARTIAL CHARATERIZATION OF ANTIBACTERIAL PEPTIDES AND PROTEINS OF THE PARATOID GLAND.
مجلة الجزيرة للعلوم الصحية, [S.l.], v. 3, n. 1, jan. 2007.
ISSN 1810-5386. متوفر في: <http://37.60.236.48/index.php/gjhs/article/view/226>. تأريخ الوصول: 03 june 2026.
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