SDS PAGE OF WHOLE CELL PROTEIN, IMMUNOBLOTTING AND PROTEIN A ASSAY FOR TYPING OF STAPHYLOCOCCUS AUREUS ISOLATED ON DOGS AND CATS
الملخص
A total of 36 isolates of Staphylococcus aureus from hospitalised and out patient dogs and cats were typed using SDS PAGE of whole cell protein, immunoblotting and protein A assayment by ELISA test. 15/24 and 14/24 profiles were recognised using SDS PAGE and immunoblotting respectively. It is concluded that SDS PAGE of whole cell protein and immunoblotting could be used as a typing methods for the characterisation of S. aureus strains. Protein A assayment could be used for the detection of S. aureus strains in samples but could not be used to differentiate between different strains.
المراجع
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Chang, T. C, and S. H. Huany (1994). An Enzyme Immunosorbent assay for the rapid detection of S. aureus in processed food. Journal of Food Protection. 57: 3, 184-189.
Costas, M., B. D. Cookson, H. G. Talsania and R. J. Owen (1989). Numerical Analysis of Electrophoric Protein Patters of methicillin-resistant strains S. aureus Journal of Clinical Microbiology. 27: 2574-2581.
Fiona, M. T. C. and T. H. Pennington (1989). Characterization of Methicillin resistant isolates of S. aureus by analysis of whole cell and exported protein. J. Medical Microbiology. 28: 25-32.
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Jaap, H. W. and H. R. Mathews (1994). The Lowery method for protein quantitation. Basic Protein and Peptide Protocol. Edited by: John M. Walker. (Methods in Molecular Biology), vol. 32: 1-6. Humana press. Totowa, New Jersey, USA.
Mullignan, M. E., R. Y. Y. Kwok, D. M. Citron, J. F. John and P. B. Smith (1988). Immunoblots, antimicrobial resistance, and bacteriophage typing of oxacillin resistant S. aureus. J. Clinical Microbiology. 26: 2395-2401.
Nei, M. and Li W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc. Natl. Acad. Science, USA. 76: 5269-5273.
Sharifah, T. (1994). Production of extracellular protein A, from a methicillin resistant strains of S. aureus. Msc. Thesis Universiti Putra Malaysia.
Tabaqchali, S., D. Holland, S. O’Farrell and R. Silman (1984). Typing scheme for Clostridium difficile: its application in clinical and epidemiological studies. Lancet. 1: 935-938.
Takeuchi, S., K. Matuda and K. Sasano (1995). Protein A in S. aureus isolated from Pigs. J. Veterinary Medical Science. 57: 581-582.
Thomson-cartre, F. M. and T. H. Pennington (1989). Characterization of methicillin-resistant isolates of staphylococcus aureus by analysis of whole-cell and exported proteins. J. Med. Microbiol. Vol. 28: 25-32.
Wantana, O. (1995). Detection of S. aureus in food by ELISA techniques. Thesis (MSc in Microbiology). Bangkok, Thailand.
Bryan, J. S. (1994). SDS Polyacrylamide gel electrophoresis of proteins. Basic Protein and Peptide Protocol. Edited by: John M. Walker. (Methods in Molecular Biology, vol 32: 1-6. Humana press. Totowa, New Jersey, USA.
Chang, T. C, and S. H. Huany (1994). An Enzyme Immunosorbent assay for the rapid detection of S. aureus in processed food. Journal of Food Protection. 57: 3, 184-189.
Costas, M., B. D. Cookson, H. G. Talsania and R. J. Owen (1989). Numerical Analysis of Electrophoric Protein Patters of methicillin-resistant strains S. aureus Journal of Clinical Microbiology. 27: 2574-2581.
Fiona, M. T. C. and T. H. Pennington (1989). Characterization of Methicillin resistant isolates of S. aureus by analysis of whole cell and exported protein. J. Medical Microbiology. 28: 25-32.
Grana, R., E. M. Fajado, N. Ruiz, E. Silva and M. T. Perez (1997). An ELISA to quantify protein A. Revista de Salud Animal. 19: 23-26.
Hollis, U. C., N. Schmeer and S. S. Newman (1986). Protein A in S. intermedius isolates from dogs and cats. American Journal of veterinary Research. 47, 9, 1881-1884.
Horacio, R., I. G. Martinez, A. T. Giraudo, A. Calzolari and R. Nagel (1996). In vivo expression of exoprotein synthesis with a sae mutant of Staphylococcus aureus. Canadian Journal of Veterinary Research. 60.237-240.
Jaap, H. W. and H. R. Mathews (1994). The Lowery method for protein quantitation. Basic Protein and Peptide Protocol. Edited by: John M. Walker. (Methods in Molecular Biology), vol. 32: 1-6. Humana press. Totowa, New Jersey, USA.
Mullignan, M. E., R. Y. Y. Kwok, D. M. Citron, J. F. John and P. B. Smith (1988). Immunoblots, antimicrobial resistance, and bacteriophage typing of oxacillin resistant S. aureus. J. Clinical Microbiology. 26: 2395-2401.
Nei, M. and Li W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc. Natl. Acad. Science, USA. 76: 5269-5273.
Sharifah, T. (1994). Production of extracellular protein A, from a methicillin resistant strains of S. aureus. Msc. Thesis Universiti Putra Malaysia.
Tabaqchali, S., D. Holland, S. O’Farrell and R. Silman (1984). Typing scheme for Clostridium difficile: its application in clinical and epidemiological studies. Lancet. 1: 935-938.
Takeuchi, S., K. Matuda and K. Sasano (1995). Protein A in S. aureus isolated from Pigs. J. Veterinary Medical Science. 57: 581-582.
Thomson-cartre, F. M. and T. H. Pennington (1989). Characterization of methicillin-resistant isolates of staphylococcus aureus by analysis of whole-cell and exported proteins. J. Med. Microbiol. Vol. 28: 25-32.
Wantana, O. (1995). Detection of S. aureus in food by ELISA techniques. Thesis (MSc in Microbiology). Bangkok, Thailand.
منشور
2008-01-01
كيفية الاقتباس
ARABI, Omer H. Arabi et al.
SDS PAGE OF WHOLE CELL PROTEIN, IMMUNOBLOTTING AND PROTEIN A ASSAY FOR TYPING OF STAPHYLOCOCCUS AUREUS ISOLATED ON DOGS AND CATS.
مجلة الجزيرة للعلوم الصحية, [S.l.], v. 4, n. 1, jan. 2008.
ISSN 1810-5386. متوفر في: <http://37.60.236.48/index.php/gjhs/article/view/224>. تأريخ الوصول: 03 june 2026.
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