Molecular characterization (PCR-Based Methods) of Staphylococcus aureus isolated on dogs and cats
Abstract
A total of 36 isolates of Staphylococcus aureus from hospitalised and out patient dogs and cats were typed by RAPD-PCR, and 36 isolates were selected for further typing by ERIC-PCR and Coagulase gene-PCR and Coagulase gene RFLP, indicating a low degree of polymorphism in the coagulase genes. In this study, it is noticeable that RAPD-PCR displayed desirable typing quality by its ability to group the apparently related isolates from outpatient and hospitalised cats and dogs, whereas ERIC-PCR has the tendency to group the isolates into a single major cluster.
References
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Altekruse, S. F., Cohen, M. L. and Swerdlow, D. C. 1997. Emerging food borne diseases. Emerging Infectious Disease 3: 285-293.
Annemuller, C., Lammler, Ch. and Zschock, M. 1999. Genotyping of Staphylococcus aureus isolated from bovine mastitis. Veterinary Microbiology 69: 217-224.
Beck, H. S., Wise W. S. and Dodd, F. H. 1992. cost benefit analysis of bovine mastitis in the UK. Journal of Dairy Research 59: 449-460.
Goh, S. H., E. E. Byrne, J. L. Zhang, and A. W. Chow (1992). Molecular typing of S. aureus on the bases of coagulase gene Polymorphism. Journal of Clinical Microbiology. 30: 1642-1645.
Hookey, J. V., J. F. Richardson and B. D. Cookson (1998). Molecular typing of based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. Journal of Clinical Microbiology. 36: 1083-1089.
Lipman, L. J., A. de Nijs, T. J. Lam, J. A. Rost, L. van Dijk, Y. H. Schakken and W. Gaastra (1996). Genotyping by PCR of S. aureus Isolated from Mammary glands of Cows. Veterinary Microbiology. 48 (1-2): 51-55.
Myllys, V., J. Ridell, J. Bjorkroth, I. Biese and S. Pyoala (1997). Persistence in Bovine Mastitis S. aureus clones as Assessed by Random Amplified Polymorphism DNA Analysis, Ribotyping and Biotyping. Veterinary Microbiology. 51: 245-251.
Nei, M. and Li W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc. Natl. Acad. Science, USA. 76: 5269-5273.
Omega, R., M. Deighton, J. Capstick and N. Gerraty (1999). Molecular typing of S. aureus of bovine origin by polymorphism of the coagulase gene. Veterinary Microbiology. 66: 275-284.
Raimundo, O., Deighton, M., Capsick, J. and N. Gerraty. 1999. Molecular typing of Staphylococcus aureus of bovine origin by polymorphism of the coagulase gene. Veterinary Microbiology 66: 275-284.
Sambrook, J., E. F. Fritsch and T. Maniatis (1989). Molecular Coloning: a Laboratory Manual, 2nd ed. Cold Spring Harbour Laboratory Press. Cold Spring Harbor. N. Y.
Saulnier, P., C. Bourneix, G. Prevost and A. Andermont (1993). Random amplified Polymorphic DNA Electrophoresis for typing strains of Methicillin-resistance S. aurues. Journal of Clinical Microbiology. 31, 4: 982-985.
Schmitz, F. J., M. Steiert, H. V. Tichy, B. Hofmann, J. Verhoef, H. P. Heinz, K. Kohrer and M. E. Jones (1998). Typing of Methicillin-resistant S. aureus isolates from Dusseldorf by six Genotyping Methods. Journal of Medical Microbiology. 47: 341-351.
Schwarzkopk, A. and K. Krach (1994). Genetic Variation in Staphylococcus aureus coagulase gene potential and limits for use as epidemiological marker. Journal of Clinical Microbiology. 32: 2407-2412.
Tenover, F. C., R. D. Arbiet, R. V. Goering, P. A. Mickelsen, B.E. Murray, D. H. Persing and B. Swaminathan (1995). Interpreting Chromosomal DNA restriction patterns produced by: pulsed field gel electrophoresis: criteria for bacterial strain typing. J. Clinical Microbiology. 33: 2233-2239.
Van-Belkum, A., R. Bax, B. B. Will, H. F. Goessen, N. van leeuwen and W. G. V. Quint (1993). Comparison of Phage Typing and DNA Fingerprinting by Polymerize Chain Reaction for Discrimination of Methicillin-resistant S. aureus strains. Journal of Clinical Microbiology. 4, 31: 798-803.
Weigler, B. J., Hird, D. W., Sischo, W. M., Holmes, J. C., Danaye-Elmi, C., Palmer, C. W. and Utterback, W. W. 1990. veterinary and non veterinary costs of disease in 29 California dairies. Journal of American Veterinary Medical Association 196: 1945-1949.
Published
2008-01-01
How to Cite
ARABI, Omer H. et al.
Molecular characterization (PCR-Based Methods) of Staphylococcus aureus isolated on dogs and cats.
Gezira Journal of Health Sciences, [S.l.], v. 4, n. 1, jan. 2008.
ISSN 1810-5386. Available at: <http://37.60.236.48/index.php/gjhs/article/view/223>. Date accessed: 03 june 2026.
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