Production and characterization of Pectic enzymes from three fungi
Abstract
Pectins are compounds that are resistant to enzymatic cleavage in
most organisms and plants, Studies were therefore made towards finding
methods for hydrolyzing these compounds. One such approach is enzymatic
hydrolysis. Three fungi (Trichoderma viride, Asperigillus niger and
penicillum digitatum) are used for production of pectinase enzymes,
Two methods (reducing sugar and viscometary) were used for measuring
enzymes activities. Pectin and sodium polypectate compounds were used as
substrates. The production of pectinases by the three fungi was investigated
in culture media. T. viride and P. digitatum gave maximum production of
pectinase enzymes after two week and A. niger gave it after one week.
Purification by ammonium sulphate precipitation, showed that the
maximum pectinase activity was at 80% concentration for both fungi A
niger and P. digitatum. By using gel electrophoresis, five bands were found
to give pectinase enzymes activities. The present study showed the
importance of fungi as sources of enzymes and recommends that more
studies must be done in the field of biotechnology to produce glucose
from natural products by using fungi.
References
caused by Xanthomonas sesame (Sabet and Dowson), Ph.D.
thesis, Department of Botany and Plant Technology, Imperial
Collage of Science and Technology. London.
Almustafa, A. A. and Abdel- Rahim, A. M. (2013). Production
and characterization of cellulolytic enzymes from three fungi.
Gez. J. of Eng. and Appl Sci. 10 (1): 1 — 19.
Ayers, W. Papavizas, GA C, and Diem, A. F. (1966),
Polygalacturonate Trans - Eleminase and Polygalacturonase
Production b Rhizoctonia solani Journal of Phytopathology. 56:
925 930.
Bateman, D and Miller, R. L. (1966}, Pectic Enzymes in Tissue
Degradation. Annual Review of Plant Pathology, 4: 119 124.
Birch, G.G, Blakebrough, N. and Parker, J. (1981). Enzyme and
Food Processing. Applied science publisher LTD„ England. Pp 5-
13.
Braverman, J. B. F. (1974) Introduction to Biochemistry of
Food. 3rd. ed, , Elsevier publishing Company. London. Pp 31.
Castaldo, D.; qualiquuolo, L; Servillo, L; Balestrieri, C. and
Giouane, A. (1989). Isolation and Characterization of
Pectinmethylesterase from Apple Fruit. Journal of Food Science,
54(3): 653 – 673.
coral, G.; B. M. Nisa, O. and Hatice, G (2001). Some
properties of Crude Carboxymethyl Cellulase of Asperigillus
niger. Turkish Journol of Biology, 26: 209 213,
Danial, J. and Emmanuel, E. (2011) , Effect of Carbon and
Nitrogen sources on Polygalacturanase Production by viride
Isolate Tar Sand in Ondo State (Nigeria). 7 (3) : 153 – 158.
Desouky, E. M, (2007), Production of cellulase by Penicillum
hordei and by Asperigillus ustus under solid state fermentation
condition, Egyptian Journal of Microbiology, 17 : 6 - 64.
Forester, H. (1988). Pectinase from Phytophthora infestants.
Journal of Enzymology. 161:307-355.
Gupta, V. K. and Ayyachamy, M. (2012), Biotechnology of
Fungal Genesc Science publisher, England. Pp 53-71.
Joshi, V. K. Mukesh, P. and Neerja, R. (2011) Purification and
Characterization of Pectinase produced from Apple Pomace and
Evaluation of its Efficacy in Fruit Juice Extraction and
Clarification. Indian Journal of Natural Products and Resources,
2(2): 189-197.
Kabli, S. A. (2007). Purification and Characterization of
Protopectinase produced by Kluyveromyces marxianus, JKAU:
Sci, vol. 19: 139 - 153.
Kareem, O. S. and Adebowalej A. A. (2002). Clarification of
Orange Juice by Crude Fungi Pectinase from Citrus Peel.
Nigerian Food Journal. 25 (1): 130 – 137.
