Determination of Gossypol Content in Cottonseed Oil and Cake by HPLC and GC/ms
Abstract
This study investigated gossypol content in cottonseed oil and cottonseed cake of Bt cottonseed (Seeni1) and non-Bt cottonseed (Barakat 90, Abdeen, Mixed) in oil and cake by difference methods. Among the all non-Bt cottonseed varieties Barakat (90) had shown higher amount of gossypol content in oil and cake (470 and 467) ppm (890 and 897) ppm by using HPLC and GC/MS respectively. Moreover, Bt cottonseed variety (Seeni1) had shown lower amount of gossypol content using both methods (320 and 326) ppm and (640 and 635) ppm by using HPLC and GC/MS respectively. From the present study, we observed that was no significant difference in gossypol content between Bt cottonseed and non-Bt cottonseed varieties. Bt cottonseed variety has lower amount of gossypol being of more economical importance to farmers having cotton as their cash crop.
References
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Kay Ledbetter, (2018). The USDA approved Texas A&M’s petition for determination of non-regulated status for Ultra-Low Gossypol Cottonseed, moving the seed one step closer to becoming a nutritious food source for millions of people. "Texas A&M Today. 2018-10-16. https://today.tamu.edu/2018/10/16/edible-cottonseed-research-at-texas-am-receives-usda-approval/
Sharief Imad, (2014). Genetically Modified (GM) Cotton is a Successful Experiment in Blue Nile, coordinator of the Sudanese- Brazilian Partnership: Sudan vision Issue 3168: 11 th February.
Sunilkumar, G., Campbell, L.M., Puckhaber, L., Stipanovic, R.D. and Rathore, K.S, (2006). Engineering cottonseed for use in human nutrition by tissue‐specific reduction of toxic gossypol. Proc. Natl. Acad. Sci. USA, pp.103, 18054– 18059.
Tian, X., Ruan, J.X., Huang, J.Q., Yang, C.Q., Fang, X., Chen, Z.W., Hong, H. et al, (2018). Characterization of gossypol biosynthetic pathway. Proc. Natl. Acad. Sci. USA, 115, E5410– E5418.
Wei Gao, Fu‐Chun Xu, Lu Long, Yang Li, Jun‐Li Zhang, Leelyn Chong, Jose Ramon Botella, Chun‐Peng Song, (2019). The gland localized CGP1 controls gland pigmentation and gossypol accumulation in cotton. https://doi.org/10.1111/pbi.13323
Zhang, W.J., Xu, Z.R., Pan, X.L., Yan, X.H. and Wang, Y.B, (2007). Advances in gossypol toxicity and processing effects of whole cottonseed in dairy cows feeding. Livest. Sci. 111, pp. 1– 9.
Alexander J., D. Benford, A. Cockburn et al., (2008). “Gossypol as undesirable substance in animal feed,” EFSA Journal, vol. 908, pp. 1–55.
Blanco B. Soto, (2008). “Gossipol e fatores antinutricionais da soja,” in Toxicologia Aplicada `a MedicIna VeterIn´aria, H. S. Spinosa, S. L. G´orniak, and J. P. Neto, Eds., pp. 531–545, Manole, Barueri, Brazil.
Ivana Cristina N. Gadelha,1 Nayanna Brunna S. Fonseca,1 Silvia Catarina S. Oloris,2 Marília M. Melo,3 and Benito Soto-Blanco3, (2014). Gossypol Toxicity from Cottonseed Products.
Jan Alexander, Diane Benford, Andrew Cockburn, and Jean-Pierre Cravedi, (2008). Scientific opinion. Gossypol as undesirable substance in animal. European Food Safety Authoirty (EFSA). EFSA J 908:1-55. [Google Scholar].
Janga, M.R., Pandeya, D., Campbell, L.M., Konganti, K., Villafuerte, S.T., Puckhaber, L., Pepper, (2019). Genes regulating gland development in the cotton plant. Plant Biotechnol. J. 17, pp.1142– 1153.
Jolanta Rubaj, Waldemar Korol and Grazyna Bielecke, (2012). Using High Perormance Liquid Chromatography (HPLC) for Analyzing Feed Additives, chapter 6, Doi: http://dx.doi.org/10.5772/47784.
Kranthi, K. R., (2012). Bt cotton Questions and answers, Published by Indian society for cotton improvement (ISCI), Mumbai.
Ma, D., Hu, Y., Yang, C., Liu, B., Fang, L., Wan, Q., Liang, W. et al, (2016). Genetic basis for glandular trichome formation in cotton.
Nicholson S. S., 2012. “Cottonseed toxicity,” in VeterInary Toxicology: Basic and Clinical Principles, R. C. Gupta, Ed., pp. 1161–1165, Academic Press, London, UK, 2nd edition.
Price W. D., R. A. Lovell, and D. G. McChesney, (2003). “Naturally occurring toxins in feedstuffs: center for veterinary medicine perspective,” Journal of Animal Science, vol. 71, no. 9, pp. 2556–2562.
Kay Ledbetter, (2018). The USDA approved Texas A&M’s petition for determination of non-regulated status for Ultra-Low Gossypol Cottonseed, moving the seed one step closer to becoming a nutritious food source for millions of people. "Texas A&M Today. 2018-10-16. https://today.tamu.edu/2018/10/16/edible-cottonseed-research-at-texas-am-receives-usda-approval/
Sharief Imad, (2014). Genetically Modified (GM) Cotton is a Successful Experiment in Blue Nile, coordinator of the Sudanese- Brazilian Partnership: Sudan vision Issue 3168: 11 th February.
Sunilkumar, G., Campbell, L.M., Puckhaber, L., Stipanovic, R.D. and Rathore, K.S, (2006). Engineering cottonseed for use in human nutrition by tissue‐specific reduction of toxic gossypol. Proc. Natl. Acad. Sci. USA, pp.103, 18054– 18059.
Tian, X., Ruan, J.X., Huang, J.Q., Yang, C.Q., Fang, X., Chen, Z.W., Hong, H. et al, (2018). Characterization of gossypol biosynthetic pathway. Proc. Natl. Acad. Sci. USA, 115, E5410– E5418.
Wei Gao, Fu‐Chun Xu, Lu Long, Yang Li, Jun‐Li Zhang, Leelyn Chong, Jose Ramon Botella, Chun‐Peng Song, (2019). The gland localized CGP1 controls gland pigmentation and gossypol accumulation in cotton. https://doi.org/10.1111/pbi.13323
Zhang, W.J., Xu, Z.R., Pan, X.L., Yan, X.H. and Wang, Y.B, (2007). Advances in gossypol toxicity and processing effects of whole cottonseed in dairy cows feeding. Livest. Sci. 111, pp. 1– 9.
Published
2021-06-03
How to Cite
EL-HASSAN, Ayia M.; M. NOUR, Salah; O.BABIKER, mohammed.
Determination of Gossypol Content in Cottonseed Oil and Cake by HPLC and GC/ms.
Gezira Journal of Engineering and Applied Sciences, [S.l.], v. 15, n. 1, p. 79-90, june 2021.
ISSN 1858-5698. Available at: <http://37.60.236.48/index.php/gjeas/article/view/1956>. Date accessed: 03 june 2026.
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