Establishment and parasitism efficiency of Trichogramma principium (Sugonyaev et Sorokina) on Helicoverpa armigera (Hb.) infesting cotton in Sudan
Abstract
This study was conducted at the Gezira Research Station (GRS) farm during 2010/11 season to verify establishment and parasitism efficiency of Trichogramma principium (Sugonyaev et Sorokina) (Hymenoptera: Trichogrammatidae) in eggs of Helicoverpa armigera (Hb.) (Lepidoptera: Noctuidae) infesting the Sudanese cotton cultivars "Barac 67B", "Hamid", "Burhan" and "Abdin". Trichogramma was acquired from the Rearing Unit, Agricultural Research Corporation (ARC), at preimaginal (prepupal) stage, in eggs of the rice moth Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae). Depending on numbers of H. armigera eggs / 100 plants, one release was done on each of Barac and Hamid and two on each of Abdin and Burhan. The release rate was 24,000 parasitoids /fed, at 7x7m distance between release points and 14-day intervals. Evaluation was done between treated plots with Trichogramma versus untreated. Observations consisted of the percentage of emerged parasitoids, percentage of parasitism and the numbers of the African bollworm larvae /100 plants. At the first release, the percentage of emerged parasitoids ranged between 71% in Barac and 86.4% in Hamid; the cultivars` average ranged between 60.5% and 94.8% and the overall average was 79.2%. The percentage of parasitized H. armigera eggs ranged between 22.2% and 60%. At the second release, the percentage of emerged adults ranged between 73.2% in Burhan and 82.1% in Abdin; the cultivars average ranged between 46.6 and 96.7% and the overall average was 77.7%. The percentage of parasitized H. armigera eggs ranged between 22.2% and 77.7%. The high level of parasitoid emergence declared a good viability of the released material, tolerance of the parasitoid to the local weather conditions and acceptance of the crop. The levels of parasitism reported were quite acceptable for this introductory release and first occurrence of the parasitoid in this new cotton agroecosysytem. A positive signal of migration from treated to untreated plots was observed through parasitized H. armigera eggs detected in the latter plots. The numbers of H. armigera larvae were negligible in both treatments. Accordingly, for proving potential capacity of establishment and parasitism efficiency against H. armigera, T. principium is strongly recommended for use on Sudanese cotton cultivars.
المقدرة على الاستيطان والكفاءة التطفلية ل Trichogramma principium (Sugonyaev et Sorokina) ضد Helicoverpa armigera (Hb.) على القطن في السودان
أجريت هذه الدراسة بمزرعة محطة بحوث الجزيرة في موسم 2010-2011 للتثبت من المقدرة على الاستيطان و الكفاءة التطفلية ل Trichogramma principium (Sugonyaev et Sorokina) (Hymenoptera: Trichogrammatidae) على بيض دودة اللوز الأفريقية Helicoverpa armigera (Hb.) (Lepidoptera: Noctuidae) على أصناف الأقطان السودانية "باراك B 67"، "حامد"، "برهان"، و"عابدين". تم الحصول على طفيل التريخوجراما من وحدة الإكثار بهيئة البحوث الزراعية في طور ماقبل التشرنق بداخل بيض فراشة الأرز Corcyra cephalonica (Lepidoptera: Pyralidae) . اعتمادا على عدد بيض دودة اللوز / 100 نبات، فلقد أجريت إطلاقه واحدة على كل من الصنفين باراك و حامد و اطلاقتان على كل من الصنفين عابدين و برهان بمعدل 24000 طفيل للفدان و على مسافات 7x7م بين نقاط الإطلاق و بفاصل 14 يوما بين الإطلاقات. تمت المقارنة بين الحقل المعامل بالتريخوجراما و الغير معامل. اشتمل التقييم على تحديد النسبة المئوية لبزوغ الأطوار المكتملة من الطفيل، النسبة المئوية للتطفل و عدد اليرقات في ال 100 نبات. عند الإطلاقة الأولى، تراوحت النسبة المئوية لبزوغ الأطوار المكتملة بين 71% كما في باراك و 86.4% كما في حامد، و المتوسط للأصناف بين 60.5% و 94.8% و المتوسط العام لهم 79.2 % و تراوحت النسبة المئوية للتطفل بين 60% و 22.2%. عند الإطلاقة الثانية، تراوحت النسبة المئوية لبزوغ الأطوار المكتملة بين 73.2%% كما في برهان و 82.1% كما في عابدين، و المتوسط للأصناف بين 46.6% و 96.7% و المتوسط العام لهم 77.7% ، و تراوحت النسبة المئوية للتطفل بين 77.7% و 22.2%. يدل هذا المستوى العالي من بزوغ الأطوار المكتملة على الحيوية الجيدة للطفيل المطلق، تحمله للظروف الجويه وتقبله للمحصول العائل. تعتبر المستويات المتحصل عليها من التطفل مناسبة لهذا الإطلاق الإستهلالى و تواجد الطفيل لأول مرة على هذا الوسط البيئي الزراعي الجديد للقطن بالسودان. هنالك مؤشرات لبداية هجرة الطفيل من المساحات المعامله لغير المعاملة تمثل في تواجد بيض متطفل علية في الأخيرة. كان عدد يرقات ديدان اللوز قليلا في المعاملتين. و عليه، و لقابليته على الاستيطان و كفاءته التطفلية المقبولة على ديدان اللوز الأفريقية فان ال T. Principium موصى باستعماله بشدة على أصناف الأقطان السودانية.
References
Abdalla, T. E. 2003. Ephestia cautella (Walker) – Trichogramma and E. cautella -Bracon hebitor Mass Rearing Program. Annual Report. Gezira Research Station, 2002 - 2003.
Abdalla, T. E. 2010. Studies on Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae), as a factitious host for Trichogramma mass rearing. Gezira Journal of Agricultural Science 8(2):1-15.
Abdalla, T. E. 2011. Trichogramma rearing technique in Sudan and effect of cool storing of host eggs on parasitism and of preimaginal parasitoids on adults` emergence. Sudan Journal of Agricultural Research (In press).
Andow, D. A. and D. R. Prokrym. 1990. Plant structural complexity and host-finding by a parasitoid. Oecologia 82: 162-165.
Basso, C. and C. Morey. 1990. Biological control of the sugarcane borer Diatraea succharalis Fab. (Lep. Pyralidae) with Trichogmmma spp. (Hym. Trichogrammatidae) in Uruguay, pp 246 In: Wajnberg, E. and S. B. Vinson. (eds.). Trichogramma and other Egg Parasitoids. Proceedings of 3rd International Symposium, San Antonio, Texas, USA.
Bigler, F. and R. Brunetti.1986. Biological control of Ostrinia nubilalis Hbn. by Trichogramma maidis Pint. et Voeg. on corn for seed production in southern Switzerland. Journal of Applied Entomology 102:303- 308.
Gross, H. R., W. J. Lewis and D. A. Nordlund. 1981. Trichogramma pretiosum: Effect of prerelease parasitization experience on retention in release areas and efficiency. Environmental Entomology 10:554-556.
Gusev, G. V. and G. I. Lebedev. 1988. Present status of Trichogramma application and research, pp. 477-483. In: Proceedings of International Symposium No. 43. Guangzhou, PR China.
Hassan, S.A. 1993. The mass rearing and utilization of Trichogramma to control lepidopterous pests: Achievements and outlook. Pesticide Science 37:387-91.
Kanour, W. W. and P. P. Burbutis. 1984. Trichogmmma nubilale (Hym: Trichogrammatidae) field releases in corn and a hypothetical model for control of European corn borer. Journal of Economic Entomology 77:103-107.
Keller, M. A., W. J. Lewis and R. E. Stinner. 1985. Biological and practical significance of movement by Trichogramma species. A review. Southwest Entomology 8:138-155.
Knipling, E. F. and J. U. McGuire. 1968. Population models to appraise the limitations and potentialities of Trichogramma in managing host insect populations. US Department of Agricultural Technologies Bulletin 1387:1-44
Li L.Y. 1994. Worldwide use of Trichogramma for biological control on different crops: A survey. pp. 37-51. In: Weinberg E. and S. A. Hassan (eds.). Biological Control with Egg Parasitoids. CAB International, Oxon, UK.
Pascua, L. T. and M. E. Pascua. 1995. Egg parasitoids of cotton bollworm, Helicoverpa armigera (Hubner) in selected cotton growing areas. Philippine Journal of Science 124: 267-273.
Prasad, C. S., M. Prasad, S. Rajendra, M. Abid Hussain, P. Rishi, K. Lomash and K. Peeyush. 2009. Highlight of Biocontrol Laboratory. Sardar Vallabh Bnai Patel University of Agriculture and Technology, Meerut, India.
Scholz, B., N. Parker and R. Lloyd. 2002. An evaluation of unsprayed INGARD strips as nurseries for beneficials in dryland cotton on the Darling Downs, pp 297-306. In: Proceedings of the Eleventh Australian Cotton Conference, Brisbane, Queensland, Australia.
Smith, M. S. 1996. Biological control with Trichogramma: Advances, success and potential of their use. Annual Review of Entomology 41: 375-406.
Smith, S. M., J. R. Carrow and J. E. Laing. 1990. Inundative release of the egg parasitoid Trichogramma minutum (Hym.: Trichogrammatidae) against forest insect pests such as the spruce budworm, Chorisroneum fumiferana (Lep.: Tortricidae). Entomological Society of Canada 153:1-87.
Suphangkasen, P. 1979. Studies on Hymenopterous parasites of Heliothis armigera eggs in Thailand. Thailand Journal of Agricultural Science 2:35-42.
Torreno, H. S. and E. P. Cadapan. 1984. Laboratory studies on two Trichogramma species against the eggs of Helicoveropa armigera (Hubner). Philippine Entomology 6(2):151-159.
Wajnberg, E. 1995. Trichogramma and other Egg Parasitoids. Les Colloques de I'INRA Paris, 226 pp.
Wang, C. L. 1988. Biological control of Ostrinia fumcalis with Trichogramma sp. in China, pp 609-613. In: Voegelt, J., J. Waage and J. C. van Lenteren (eds.) Trichogramma and other Egg Parasites. 2nd International Symposium on Trichogramma. Guangzhou, PR China.
Witz, J. A., A. W. Hartstack, E. G. King, W. A. Dickerson and J. R. Phillips. 1985. Monitoring and prediction of Heliothis spp. Southwest Entomology 8:56-71.
Yu, D. S. and J. R. Byers. 1994. Inundative release of Trichogramma brarsicae Bezdenko (Hym.: Trichogrammatidae) for control of European corn borer in sweet corn. Canada Entomology 126:291-301.
