Embryoid induction and plantlet regeneration from leaf segments of sugarcane (Saccharum officinarum L.)
Abstract
Sugarcane (Saccharum officinarum L.) is an economically important crop in Sudan for domestic consumption and export. It is the first and essential source for production of high purity sugar. Tissue culture techniques can be used for in vitro conservation of sugarcane (Taylor and Dukic, 1993) and mass propagation of elite cultivars of crop species. Moreover, it was used for production of pathogen-free planting material from infected mother plants. Variability induced in vitro can furnish a base for improvement of vegetatively propagated crops including sugarcane. Different tissue culture techniques were applied successfully to sugarcane propagation and plant regeneration through organogenesis of shoot meristem (Nadar and Heinz, 1977; Ho and Vasil, 1983a), cell suspension cultures (Ho and Vasil, 1983b, Aftab et al., 1996) and protoplast cultures ( Liu, 1994). Cell suspen - Sion cultures were also used for cytological, pathological (Peros and Lombard, 1992), biochemical and physiological investigations of sugarcane (Heinz et al., 1977).
This study was initiated, during 1998-2000, to investigate the effect
of 2,4-D on induction of embryogenic callus from leaf explants of sugarcane and regeneration of somatic embryos on different concen-trations of Murashige and Skoog (1962) medium (MS).
References
Heinz, D.Jo, M. Klishnamurthi, L. G. Nickell and A. Maretzki. 1977. Cell tissue and organ culture in sugarcane improvemnet, pp. 3-17. In: J. Reinert and Y. P. S. Bajaj (eds). Applied and Fundamental Aspects of Plant Cell and Organ Culture. Springer-Verlag, Berlin,
Germany.
Ho, W J. and I. K. Vasil. 1983a. Somatic embryogenic in sugarcane (Saccharum officinarum L.) growth and plant regeneration from embryogenic and suspension cultures. Annals of Botany 51:719- 726.
Ho, W.J. and 1K. Vasili 1983b. Somatic embryogenic in sugarcane (Saccharum officinarum L). The morphology and physiology of callus formation and the ontogeny of somatic embryoso Proto- plasma 118: 169-180.
Grisham Me P. and D. Bourg. 1989. Efficiency of in vitro propagation of sugarcane plants by direct regeneration from leaf tissue and by
shoot tip culture. American Society for Sugarcane Technology 9:
97-102.
Liu, M. C. 1994. A novel method of plant regeneration from suspe- nsion culture protoplasts of sugarcane. Journal of Plant Physiology 143: 753-755.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid gowth and bioassay with tobacco tissue culture. Physiologia Plantarum 15: 473-479.
Nadar, H. M. and J. D. Heinz .1977. Root and shoot development from sugarcane callus tissue. Crop Science 17: 814-816.
Peros, J. P., E. Bonnel and B. Reynaud. 1990. In vitro culture of sugarcane infected with maize streak virus. Plant Cell, Tissue and Organ Culture 29: 145-151.
Peros, J. and H. Lombard. 1992. In vitro evaluation of sugarcane resistance to gumming disease and of Xanthomonas campestris pv. vasculorum aggressiveness. Plant Cell, Tissue and Organ Culture
29: 145-151.
Sheng, T. and G. Lee. 1987. Micropropagation of Sugarcane. Plant
Cell, Tissue and Organ Culture 10:47-55.
Taylor, P. W. J. and S. Dukic. 1993. Development of an in vitro culture technique for conservation of Saccharum spp. Hybrid germplasm. Plant Cell, Tissue and Organ Culture 34: 217 222.
vasil, V., C. Lu, P. Oziaé-Akins, Z. Haydu and D. Y. Wang. 1982. Somatic embryogenesis, pp. 3-21. In: E. Earle and Y. Demarly (eds). Cereals and Grasses. Praeger Press. New York, U.S.A.
