Effect of cytokinins and auxins on micropropagation of shoot tip and nodal explants of two cultivars of sweet potato (Ipomoea batatas (L) Lam)
الملخص
In vitro propagation of two selected cultivars of sweet potato (Ipomoea batatas (L) Larn), Baladi White (BW) and Nigerian (N) were investigated using shoot tips and nodal explants during the period March, 1997 to July, 1998. Shoot regeneration from both cultivars was best on Murashige and Skoog (1962) medium without plant growth regulators. Morphogenetic response varied with the different types of explants and genotypes of sweet potato. Shoot morphogenesis from the sweet potato cv. BW was better than that from cv. N. Shoot tip explant was better for in vitro propagation of sweet potato cv. N. , while nodal cuttings were. better for cv. BW. The shoot regeneration rate induced on benzylaminopurine (BAP) was higher than that on kinetin. BAP in combination with naphthalene acetic acid (NAA) resulted in shoot and root morphogenesis from nodal explants of the sweet potato cv. N. The best shoot length was found on Murashige and Skoog medium (MS) supplemented with NAA at both 0.25 and 1.0 mg/l combined with 0.5 mg/l BAP.
المراجع
Meristem tip culture and virus indexing of sweet potatoes. Phyto-
pathology 65: 769-773.
Bouwkamp, J.C. 1985. Sweet Potato Production, a Natural Resource
for the Tropics, CPC Press, Boca Raton, Florida, U.S.A.
FAO .1999. Dossier, roots and tubers. "Their role in food security".
The Courier No. 101 A :62-65, FAO, Rome.
Gousukonda, R.M., CS. Prakash and A.P. Dessai. 1995. Shoot rege-
neration in vitro from diverse genotypes of sweet potato and
multiple shoot production per explante HortScience 30 (5):1077.
Hall, M. R. and S. C Phatak. 1993. Sweet potato, pp. 693-707. In: G.
Kalloo and B. O. Berg ( eds). Genetic Improvement of Vegetable
Crops. Pergamon Press, Oxford, U.K.
Kriuk, No, A. Supat and V. Rongong.1988; Proceedings of.the Sixth
Methodological Techniques in Biological Sciences, pp. 66-67.
Research and Development Institute, Kasetsart University, Nakhen
Pathon, Thailand.
Liao, CH. and Me L. Chung.1979. Shoot tip culture and virus inde-
Xing in sweet potato. Journal of Agricultural Research of China
28:139-144.
Litz, R.Eø and R. A. Conover. 1978. In vitro propagation of sweet
potato. HortScience 13: 659-660.
Liu, J.R. and D. J. Cantliffe. 1984 Somatic embryogenesis and plant
regeneration in tissue culture of sweet potato. Plant Cell Report 3:
112-115.
Murashige, and F. Skoog. 1962. A revised medium for rapid
growth and bioassay with tobacco tissue cultures. Physiologia
Plantarum 15:473-497.
MSU. 1993. MSTAT-C microcomputer programme for the desiY1,
management and analysis of agronomic research experiments.
Michigan State University, East Liasing, Michigan, USA.
Santangelo, E. and A. Sonnino. 1991. In vitro and in vivo vegetative
propagation of sweet potato (Ipomoea batatas L). Tropical Science
85(1): 39-52
Shimonishi, Ka, M. Karube and HO Kukimura. 1992. Rapid embryo-
genesis by NAA and plant regeneration in sweet potato. Japanese
Journal of Breeding 42:60-61.
Yamaguchi, T. and T. Nakajima. 1972. Effect of abscisic acid on
adventitious bud formation from cultured *tissue of sweet potato.
Crop Science Society of Japan 41: 531-532.
