Effect of adenine sulfate, benzylaminopurine and media forms on propagation of banana (Musa AAA) cv. Gros Michel and plantain (Musa ABB) cv. Cardaba
الملخص
This study was initiated to test the effect of adenine sulfate, benzylaminopurine (BAP) and media forms on in vitro morphogenesis of banana cv. Gros Michel and plantain cv; Cardaba during the period January, 2001 to May, 2002. The number of plantlets per explant increased significantly on both cultivars cultured on liquid prop- agation medium with filter paper bridge compared to other forms of media. However, the percentage of explants with shoot regeneration was comparable on all treatments. Comparable percentage of shoot tip explants with shoots were induced from cv. Gros Michel culture on different concentrations of adenine sulfate and the propagation medium which represented the control. However, the number of shoots per explant decreased significantly except that on 100mg/l which was comparable with the propagation medium. The percentage of explants with roots and number of roots per explant increased significantly on cv. Gros Michel on adenine sulfate compared with the propagation medium. The different concentrations of adenine sulfate induced comparable percentage of explants with roots and shoots on cv. Cardaba. However, the number of explants with shoots induced on Cardaba decreased significantly when adenine sulfate was added to the propagation medium and the number of roots per explant increased significantly compared with the control. Benzylaminopurine induced similar percentages of explants with shoot regeneration on cvs. Gros Michel and Cardaba. Significantly higher number of shoots per explants of banana cv. Gros Michel was induced on mediun supplemented with BAP at 5 or 7.5 mg/l. However, the highest nurnber of shoots regenerated from plantain cv. Cardaba was induced
by higher concentrations of BAP (7.5 and 10.0 mg/l). There were genotypic differences in the response of the two cultivars to BAP.
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